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Anti-CK10/Cytokeratin 10 Antibody (1G407)
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Catalog No. TMAB-04382
Anti-CK10/Cytokeratin 10 Antibody (1G407) is a Rabbit antibody targeting CK10/Cytokeratin 10. Anti-CK10/Cytokeratin 10 Antibody (1G407) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CK10/Cytokeratin 10 Antibody (1G407)
Anti-CK10/Cytokeratin 10 Antibody (1G407)
Copy Product Info😃Good
Catalog No. TMAB-04382
Anti-CK10/Cytokeratin 10 Antibody (1G407) is a Rabbit antibody targeting CK10/Cytokeratin 10. Anti-CK10/Cytokeratin 10 Antibody (1G407) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CK10/Cytokeratin 10 Antibody (1G407) is a Rabbit antibody targeting CK10/Cytokeratin 10. Anti-CK10/Cytokeratin 10 Antibody (1G407) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 1G407 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei. 6. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei. 7. Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei. 8. 25 μg total protein per lane of various lysates (see on figure) probed with CK10/Cytokeratin 10 monoclonal antibody, unconjugated (TMAB-04382) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 9. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK10/Cytokeratin 10 Monoclonal Antibody, Unconjugated (TMAB-04382) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:1000-10000; IHC-P: 1:1000-5000; IHC-Fr: 1:1000-5000; IF: 1:1000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Seen in all suprabasal cell layers including stratum corneum. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. |
| Immunogen | A synthesized peptide: human Cytokeratin 10 |
| Antigen Species | Human |
| Gene Name | KRT10 |
| Gene ID | |
| Protein Name | Keratin, type I cytoskeletal 10 |
| Uniprot ID |
| Molecular Weight | Theoretical: 59 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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