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Anti-CENPC Antibody (3X906)

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Catalog No. TMAC-00815
Alias MIF2, Interphase centromere complex protein 7, ICEN7, hcp-4, Centromere protein C 1, Centromere protein C, Centromere autoantigen C1, Centromere autoantigen C, CENPC1, CENPC_HUMAN, CENP-C 1, CENP-C

Anti-CENPC Antibody (3X906) is a Rabbit antibody targeting CENPC. Anti-CENPC Antibody (3X906) can be used in WB,ICC,IF,IHC,FCM,IP.

Anti-CENPC Antibody (3X906)

Anti-CENPC Antibody (3X906)

😃Good
Catalog No. TMAC-00815Alias MIF2, Interphase centromere complex protein 7, ICEN7, hcp-4, Centromere protein C 1, Centromere protein C, Centromere autoantigen C1, Centromere autoantigen C, CENPC1, CENPC_HUMAN, CENP-C 1, CENP-C
Anti-CENPC Antibody (3X906) is a Rabbit antibody targeting CENPC. Anti-CENPC Antibody (3X906) can be used in WB,ICC,IF,IHC,FCM,IP.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-CENPC Antibody (3X906) is a Rabbit antibody targeting CENPC. Anti-CENPC Antibody (3X906) can be used in WB,ICC,IF,IHC,FCM,IP.
SynonymsMIF2, Interphase centromere complex protein 7, ICEN7, hcp-4, Centromere protein C 1, Centromere protein C, Centromere autoantigen C1, Centromere autoantigen C, CENPC1, CENPC_HUMAN, CENP-C 1, CENP-C
Ig Type
IgG
Clone
3X906
Reactivity
Human
Verified Activity
1. Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
3. Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
4. ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundA replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: human CENPC aa 400-600
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 107 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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