Anti-CDKN1B Polyclonal Antibody is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Polyclonal Antibody can be used in FCM,IF,IHC-Fr,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Sheep)

1. Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
2. CDKN1B (p27Kip1) was immunoprecipitated from mouse kidney tissue with TMAB-00410 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Goat anti-Rabbit IgG (Heavy Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: mouse kidney tissue lysate 10 µg (Input).
Lane 2: TMAB-00410 IP in mouse kidney tissue lysate.
Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control).
Secondary: Goat anti-Rabbit IgG (Heavy Chain specific), HRP Conjugated, 1:5000
3. Sample:
293t (Human) Cell Lysate at 30 μg
U2os (Human) Cell Lysate at 30 μg
Primary: Anti-CDKN1B/p27 KIP 1 (TMAB-00410) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:200-800; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human P27 kip1

Human

CDKN1B

Cyclin-dependent kinase inhibitor 1B

Cip/Kip,Cip / Kip,Cip/kip

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.