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Anti-CDKN1B Polyclonal Antibody

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Catalog No. TMAB-00410

Anti-CDKN1B Polyclonal Antibody is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-CDKN1B Polyclonal Antibody

Anti-CDKN1B Polyclonal Antibody

😃Good
Catalog No. TMAB-00410
Anti-CDKN1B Polyclonal Antibody is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CDKN1B Polyclonal Antibody is a Rabbit antibody targeting CDKN1B. Anti-CDKN1B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Sheep)
Verified Activity
1. Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
2. Tissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated (TMAB-00410) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. CDKN1B (p27Kip1) was immunoprecipitated from mouse kidney tissue with TMAB-00410 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Goat anti-Rabbit IgG (Heavy Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: mouse kidney tissue lysate 10 µg (Input).
Lane 2: TMAB-00410 IP in mouse kidney tissue lysate.
Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control).
Secondary: Goat anti-Rabbit IgG (Heavy Chain specific), HRP Conjugated, 1:5000
4. Sample:
293t (Human) Cell Lysate at 30 μg
U2os (Human) Cell Lysate at 30 μg
Primary: Anti-CDKN1B/p27 KIP 1 (TMAB-00410) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human P27 kip1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cip/Kip,Cip / Kip,Cip/kip
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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