Anti-CD9 Polyclonal Antibody 3 is a Rabbit antibody targeting CD9. Anti-CD9 Polyclonal Antibody 3 can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Rat,Cow,Rabbit,Sheep)

1. Blank control: MCF-7.
Primary Antibody (green line): Rabbit Anti-CD9 antibody (TMAB-04095)
Dilution: 1 μg/Test;
Secondary Antibody (green line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD9) Polyclonal Antibody, Unconjugated (TMAB-04095) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD9

Human

CD9

CD9 antigen

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.