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Anti-CD8 alpha Polyclonal Antibody is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Polyclonal Antibody can be used in FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-CD8 alpha Polyclonal Antibody is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Polyclonal Antibody can be used in FCM. |
Ig Type | IgG |
Reactivity | Human (predicted:Mouse) |
Verified Activity | Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-CD8 antibody (TMAB-00388) Dilution: 2 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | FCM |
Recommended Dose | FCM: 2ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane; Single-pass type I membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020] |
Immunogen | KLH conjugated synthetic peptide: mouse CD8 |
Antigen Species | Mouse |
Gene ID | |
Uniprot ID | |
Biology Area | Cytotoxic Cells,CD,CD markers |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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