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Anti-CD8 alpha Polyclonal Antibody

😃Good
Catalog No. TMAB-00388
Alias Lyt-2, Ly-B, Ly-35, Ly-2, CD8a molecule, CD8 α, BB154331

Anti-CD8 alpha Polyclonal Antibody is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Polyclonal Antibody can be used in FCM.

Anti-CD8 alpha Polyclonal Antibody

Anti-CD8 alpha Polyclonal Antibody

😃Good
Catalog No. TMAB-00388Alias Lyt-2, Ly-B, Ly-35, Ly-2, CD8a molecule, CD8 α, BB154331
Anti-CD8 alpha Polyclonal Antibody is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Polyclonal Antibody can be used in FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-CD8 alpha Polyclonal Antibody is a Rabbit antibody targeting CD8 alpha. Anti-CD8 alpha Polyclonal Antibody can be used in FCM.
AliasLyt-2, Ly-B, Ly-35, Ly-2, CD8a molecule, CD8 α, BB154331
Ig Type
IgG
Reactivity
Human (predicted:Mouse)
Verified Activity
Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-CD8 antibody (TMAB-00388)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
Recommended Dose
FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse CD8
Antigen Species
Mouse
Gene ID
Uniprot ID
Biology Area
Cytotoxic Cells,CD,CD markers
Chemical Properties
Molecular WeightTheoretical: 27 kDa. Actual: 32 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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