Anti-CD58 Polyclonal Antibody is a Rabbit antibody targeting CD58. Anti-CD58 Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Sample: Raji Cell lysate at 30 μg;
Primary: Anti-CD58/LFA3 (TMAB-04031) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000 dilution;
Predicted band size: 25 kD
Observed band size: 50 kD
2. Blank control: K562.
Primary Antibody (green line): Rabbit Anti-CD58 antibody (TMAB-04031)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD58

Human

CD58

Lymphocyte function-associated antigen 3

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.