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Anti-CD44 Antibody (7A260) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (7A260) can be used in WB,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $426 | 7-10 days |
Description | Anti-CD44 Antibody (7A260) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (7A260) can be used in WB,IHC,FCM. |
Synonyms | Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group) |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of CD44 on different cell lysates using anti- CD44 antibody at 1/500 dilution. Positive control: Line1: HUVEC, Line2: HL-60, Line3: Hela, Line4: SKOV3. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD44 antibody. Counter stained with hematoxylin. 4. Flow cytometric analysis of Hela cells with CD44 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:200; FCM: 1:100-200 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | Peptide affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | CD44 is a cell-surface glycoprotein postulated to play a role in a variety of biological processes, including lymphocyte homing and tumor-cell metastasis. It mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA), and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications. |
Conjucates | Unconjugated |
Immunogen | Peptide |
Uniprot ID |
Molecular Weight | Theoretical: 80 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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