Anti-CD38 Antibody (3T794) is a Mouse antibody targeting CD38. Anti-CD38 Antibody (3T794) can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF,FCM.

IgG1

3T794

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (TMAB-03968) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (TMAB-03968) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (TMAB-03968) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (TMAB-03968) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD38 Monoclonal Antibody, Unconjugated (TMAB-03968) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (CD38) monoclonal Antibody, unconjugated (TMAB-03968) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
7. The Raji (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-CD38 antibody (TMAB-03968): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
8. 25 μg total protein per lane of various lysates (see on figure) probed with CD38 monoclonal antibody, unconjugated (TMAB-03968) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:50-200; FCM: 1μg/Test

Monoclonal

Recombinant Protein: human CD38 protein

Human

CD38

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1

Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.