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Anti-CD28 Polyclonal Antibody

😃Good
Catalog No. TMAB-00349

Anti-CD28 Polyclonal Antibody is a Rabbit antibody targeting CD28. Anti-CD28 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-CD28 Polyclonal Antibody

Anti-CD28 Polyclonal Antibody

😃Good
Catalog No. TMAB-00349
Anti-CD28 Polyclonal Antibody is a Rabbit antibody targeting CD28. Anti-CD28 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CD28 Polyclonal Antibody is a Rabbit antibody targeting CD28. Anti-CD28 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Reactivity
Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse)
Verified Activity
1. Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated (TMAB-00349) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated (TMAB-00349) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Positive control: (mo) splenocytes (2% Paraformaldehyde-fixed)
Isotype Control Antibody
Antibody: Rabbit IgG;
Supplier: Cell Signaling Technology;
Dilution: 1 μg in 100 μL 1 X PBS containing 0.5% BSA
Secondary Antibody
Antibody: Goat anti-rabbit IgG-FITC;
Supplier: Cell Signaling Technology;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody
Supplier: Bioss;
Supplier catalog number: TMAB-00349;
Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA
4. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (TMAB-00349) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse CD28
Antigen Species
Mouse
Gene ID
Uniprot ID
Biology Area
CD,Regulatory T Cells,CD,NK Cell Lineage,T Lymphocytic Lineage
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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