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Anti-CD24 Polyclonal Antibody

😃Good
Catalog No. TMAB-00347
Alias MGC75043, FLJ43543, FLJ22950, CD24A, CD24 molecule, CD 24

Anti-CD24 Polyclonal Antibody is a Rabbit antibody targeting CD24. Anti-CD24 Polyclonal Antibody can be used in WB,FCM.

Anti-CD24 Polyclonal Antibody

Anti-CD24 Polyclonal Antibody

😃Good
Catalog No. TMAB-00347Alias MGC75043, FLJ43543, FLJ22950, CD24A, CD24 molecule, CD 24
Anti-CD24 Polyclonal Antibody is a Rabbit antibody targeting CD24. Anti-CD24 Polyclonal Antibody can be used in WB,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-CD24 Polyclonal Antibody is a Rabbit antibody targeting CD24. Anti-CD24 Polyclonal Antibody can be used in WB,FCM.
AliasMGC75043, FLJ43543, FLJ22950, CD24A, CD24 molecule, CD 24
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Sample:
Colon carcinoma (Human) lysate at 30 μg;
Heart (Rat) lysates, 30 μg;
Primary: Anti-CD24 (TMAB-00347) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 3000;
Predicted band size: 8 kDa
Observed band size: 35 kDa
2. Blank control: A549 (blue). Primary Antibody: Rabbit Anti-CD24 antibody (TMAB-00347), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00347, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
Application
WB,FCM
Recommended Dose
WB: 1:500-2000; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CD24 Propeptide
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cellular metabolic process,Response to hypoxia,Tumor antigens,CD,Hypoxia,Neural Stem Cell marker,Human Lineage Negative,B Lymphocytic Lineage,T Lymphocytic Lineage,Dendritic Cell Lineage,Erythrocytic Lineage,Monocytic Lineage,Neutrophil Lineage
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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