Anti-CD24 Polyclonal Antibody is a Rabbit antibody targeting CD24. Anti-CD24 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Sample:
Colon carcinoma (Human) lysate at 30 μg;
Heart (Rat) lysates, 30 μg;
Primary: Anti-CD24 (TMAB-00347) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 3000;
Predicted band size: 8 kDa
Observed band size: 35 kDa
2. Blank control: A549 (blue). Primary Antibody: Rabbit Anti-CD24 antibody (TMAB-00347), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00347, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human CD24 Propeptide

Human

Cellular metabolic process,Response to hypoxia,Tumor antigens,CD,Hypoxia,Neural Stem Cell marker,Human Lineage Negative,B Lymphocytic Lineage,T Lymphocytic Lineage,Dendritic Cell Lineage,Erythrocytic Lineage,Monocytic Lineage,Neutrophil Lineage