Anti-CD20 Antibody (5B572) is a Mouse antibody targeting CD20. Anti-CD20 Antibody (5B572) can be used in IHC-P,WB,IHC-Fr,IF.

IgG

5B572

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with CD20 monoclonal antibody, unconjugated (TMAB-03905) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
9. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Cerebrum;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (TMAB-03905) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.

IHC-P: 1:100-500; WB: 1:500-2000; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human CD20

Human

MS4A1

B-lymphocyte antigen CD20

This protein may be involved in the regulation of B-cell activation and proliferation