Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Human,Mouse

IgG

9P745

Human,Mouse

1. Sample:
Lane 1: mouse thymus tissue lysate
Primary: Anti-RANTES (TMAB-00315) at 1:1000 dilution
Secondary: Goat Anti-Rabbit IgG-HRP at 1:20000 dilution
Predicted band size: 7.4/10 kDa
Observed band size: 15 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RANTES) Monoclonal Antibody, Unconjugated (TMAB-00315) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human lung caner); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RANTES) Monoclonal Antibody, Unconjugated (TMAB-00315) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (RANTES) Monoclonal Antibody, Unconjugated (TMAB-00315) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (RANTES) monoclonal Antibody, Unconjugated (TMAB-00315) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
6. HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (RANTES) monoclonal Antibody, Unconjugated (TMAB-00315) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB,IHC-P,IHC-Fr,ICC/IF,IF

WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50; IF: 1:50-200

Monoclonal

KLH conjugated synthetic peptide: human RANTES

Human

6352