Anti-CCKBR Polyclonal Antibody is a Rabbit antibody targeting CCKBR. Anti-CCKBR Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse,Rat (predicted:Human,Cow,Rabbit)

1. Sample: Stomach (Mouse) Lysate at 40 μg
Primary: Anti-CCKBR (TMAB-00304) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 53 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCKBR) Polyclonal Antibody, Unconjugated (TMAB-00304) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-CCKBR (TMAB-00304) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Gastrin receptor

Human

CCKBR

Gastrin/cholecystokinin type B receptor

Obesity,Hypothalamic pituitary adrenal axis,More GPCR

Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.