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Anti-CCKBR Polyclonal Antibody is a Rabbit antibody targeting CCKBR. Anti-CCKBR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-CCKBR Polyclonal Antibody is a Rabbit antibody targeting CCKBR. Anti-CCKBR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Gastrin\cholecystokinin brain receptor, Gastrin/cholecystokinin type B receptor, Gastrin receptor, GASR, CholecystokininB receptor, Cholecystokinin2 receptor, Cholecystokinin B receptor, Cholecystokinin 2 receptor, CCKRB, CCKR Type B, CCK-BR, CCKBR, CCK-B receptor, CCKB receptor, CCKB, CCK2-R, CCK2 receptor, CCK2 R, CCK BR, CCK B receptor, CCK B |
Ig Type | IgG |
Reactivity | Mouse,Rat (predicted:Human,Cow,Rabbit) |
Verified Activity | 1. Sample: Stomach (Mouse) Lysate at 40 μg Primary: Anti-CCKBR (TMAB-00304) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 53 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCKBR) Polyclonal Antibody, Unconjugated (TMAB-00304) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Cerebellum (Mouse) Lysate at 40 μg Primary: Anti-CCKBR (TMAB-00304) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism. |
Immunogen | KLH conjugated synthetic peptide: human Gastrin receptor |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Obesity,Hypothalamic pituitary adrenal axis,More GPCR |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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