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Anti-Caspase-8 Antibody (7P665)
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Catalog No. TMAB-00289
Alias MORT1-associated ced-3 homolog (MACH), MCH5, ICE-like apoptotic protease 5, FADD-like ICE (FLICE), FADD-homologous ICE/ced-3-like protease, Caspase-8, CASP-8, CASP8, CAP4, Apoptotic protease Mch-5, Apoptotic cysteine protease
Anti-Caspase-8 Antibody (7P665) is a Mouse antibody targeting Caspase-8. Anti-Caspase-8 Antibody (7P665) can be used in WB.
Anti-Caspase-8 Antibody (7P665)
Anti-Caspase-8 Antibody (7P665)
😃Good
Catalog No. TMAB-00289Alias MORT1-associated ced-3 homolog (MACH), MCH5, ICE-like apoptotic protease 5, FADD-like ICE (FLICE), FADD-homologous ICE/ced-3-like protease, Caspase-8, CASP-8, CASP8, CAP4, Apoptotic protease Mch-5, Apoptotic cysteine protease
Anti-Caspase-8 Antibody (7P665) is a Mouse antibody targeting Caspase-8. Anti-Caspase-8 Antibody (7P665) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-Caspase-8 Antibody (7P665) is a Mouse antibody targeting Caspase-8. Anti-Caspase-8 Antibody (7P665) can be used in WB. |
| Alias | MORT1-associated ced-3 homolog (MACH), MCH5, ICE-like apoptotic protease 5, FADD-like ICE (FLICE), FADD-homologous ICE/ced-3-like protease, Caspase-8, CASP-8, CASP8, CAP4, Apoptotic protease Mch-5, Apoptotic cysteine protease |
| Ig Type | IgG |
| Clone | 7P665 |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg Jurkat (Human) Cell Lysate at 30 μg Molt-4 (Human) Cell Lysate at 30 μg K562 (Human) Cell Lysate at 30 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 43 kDa 2. Sample: Stomach (Mouse) Lysate at 40 μg Spleen (Mouse) Lysate at 40 μg LympHnode (Mouse) Lysate at 40 μg Urinary bladder (Mouse) Lysate at 40 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 55/53D 3. Sample: 293T-Uv (Human) Cell Lysate at 30 μg U251-Uv (Human) Cell Lysate at 30 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 43 kDa |
| Application | |
| Recommended Dose | WB: 1:2000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. |
| Immunogen | Recombinant Protein: human Caspase 8 Protein |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Caspases,Metabolism,Necroptosis,Caspases,Caspases,Other proteases,Apoptosis |
| Molecular Weight | Theoretical: 43/57 kDa. Actual: 43 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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