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Anti-Caspase-8 Antibody (7P665)
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Catalog No. TMAB-00289
Alias MORT1-associated ced-3 homolog (MACH), MCH5, ICE-like apoptotic protease 5, FADD-like ICE (FLICE), FADD-homologous ICE/ced-3-like protease, Caspase-8, CASP-8, CASP8, CAP4, Apoptotic protease Mch-5, Apoptotic cysteine protease
Anti-Caspase-8 Antibody (7P665) is a Mouse antibody targeting Caspase-8. Anti-Caspase-8 Antibody (7P665) can be used in WB.
Anti-Caspase-8 Antibody (7P665)
Anti-Caspase-8 Antibody (7P665)
😃Good
Catalog No. TMAB-00289Alias MORT1-associated ced-3 homolog (MACH), MCH5, ICE-like apoptotic protease 5, FADD-like ICE (FLICE), FADD-homologous ICE/ced-3-like protease, Caspase-8, CASP-8, CASP8, CAP4, Apoptotic protease Mch-5, Apoptotic cysteine protease
Anti-Caspase-8 Antibody (7P665) is a Mouse antibody targeting Caspase-8. Anti-Caspase-8 Antibody (7P665) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Caspase-8 Antibody (7P665) is a Mouse antibody targeting Caspase-8. Anti-Caspase-8 Antibody (7P665) can be used in WB. |
| Synonyms | MORT1-associated ced-3 homolog (MACH), MCH5, ICE-like apoptotic protease 5, FADD-like ICE (FLICE), FADD-homologous ICE/ced-3-like protease, Caspase-8, CASP-8, CASP8, CAP4, Apoptotic protease Mch-5, Apoptotic cysteine protease |
| Ig Type | IgG |
| Clone | 7P665 |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg Jurkat (Human) Cell Lysate at 30 μg Molt-4 (Human) Cell Lysate at 30 μg K562 (Human) Cell Lysate at 30 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 43 kDa 2. Sample: Stomach (Mouse) Lysate at 40 μg Spleen (Mouse) Lysate at 40 μg LympHnode (Mouse) Lysate at 40 μg Urinary bladder (Mouse) Lysate at 40 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 55/53D 3. Sample: 293T-Uv (Human) Cell Lysate at 30 μg U251-Uv (Human) Cell Lysate at 30 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 43 kDa |
| Application | |
| Recommended Dose | WB: 1:2000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. |
| Immunogen | Recombinant Protein: human Caspase 8 Protein |
| Antigen Species | Human |
| Gene Name | CASP8 |
| Gene ID | |
| Protein Name | Caspase-8 |
| Uniprot ID | |
| Biology Area | Caspases,Metabolism,Necroptosis,Caspases,Caspases,Other proteases,Apoptosis |
| Function | Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. |
| Molecular Weight | Theoretical: 43/57 kDa. Actual: 43 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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