Anti-CASP4 Polyclonal Antibody 2 is a Rabbit antibody targeting CASP4. Anti-CASP4 Polyclonal Antibody 2 can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Rabbit,Zebrafish)

1. Blank control: U937.
Primary Antibody (green line): Rabbit Anti-Caspase 4 p20 antibody (TMAB-03642)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse Caspase-4 subunit p20

Mouse

CASP4

Caspase-4

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1.