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Anti-C9orf3 Polyclonal Antibody

Catalog No. TMAB-03512 Copy Product Info
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Anti-C9orf3 Polyclonal Antibody is a Rabbit antibody targeting C9orf3. Anti-C9orf3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-C9orf3 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-03512

Anti-C9orf3 Polyclonal Antibody is a Rabbit antibody targeting C9orf3. Anti-C9orf3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-C9orf3 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-C9orf3 Polyclonal Antibody is a Rabbit antibody targeting C9orf3. Anti-C9orf3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Chicken)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C9orf3) Polyclonal Antibody, Unconjugated (TMAB-03512) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Placenta (Mouse) Lysate at 40 μg
Primary: Anti-C9orf3 (TMAB-03512) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kD
Observed band size: 94 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm (Potential).
Tissue SpecificityPredominantly expressed in pancreas, placenta, liver, testis and heart. Expressed at lower level in brain, lung and kidney.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Concentration1mg/ml
Research BackgroundC9orf3 is an aminopeptidase and belongs to the M1 family of metallopeptidases or gluzincins. Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. C9orf3 is able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway but not able to cleave angiotensin I and angiotensin II. It may play a role in the proteolytic processing of bioactive peptides in tissues where it is expressed. C9orf3 is predominantly expressed in pancreas, placenta, liver, testis and heart and at lower level in brain, lung and kidney. There are four named isoforms.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human C9orf3
Antigen Species
Human
Gene Name
AOPEP
Gene ID
Protein Name
Aminopeptidase O
Uniprot ID
Function
Aminopeptidases catalyze the hydrolysis of amino acid residues from the N-terminus of peptide or protein substrates. Able to cleave angiotensin III to generate angiotensin IV, a bioactive peptide of the renin-angiotensin pathway. Not able to cleave angiotensin I and angiotensin II. May play a role in the proteolytic processing of bioactive peptides in tissues such as testis and heart.
Chemical Properties
Molecular WeightTheoretical: 94 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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