Anti-C5a Polyclonal Antibody is a Rabbit antibody targeting C5a. Anti-C5a Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat)

1. Sample: HepG2 (human) Lysate at 40 μg
Primary: Anti-C5 (TMAB-00262) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 104/112 kDa
Observed band size: 117 kDa
2. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-C5 antibody (TMAB-00262)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-C5a (TMAB-00262) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115/105 kDa
Observed band size: 115 kDa

WB,FCM

WB: 1:500-2000; FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Complement C5 alpha chain

Human

Oxidative Stress,Alternative Pathway,Classical Pathway,MAC,Macrophage / Inflamm.,Oxidative stress,GPCR