Anti-C4orf52 Polyclonal Antibody is a Rabbit antibody targeting C4orf52. Anti-C4orf52 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Rabbit,Sheep)

1. Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-C4orf52 Polyclonal Antibody, Unconjugated (TMAB-03449) 1:5000, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
2. Sample: muscle (Mouse) Lysate at 40 μg
Primary: Anti-C4orf52 (TMAB-03449) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
3. Sample: heart (Mouse) Lysate at 40 μg
Primary: Anti-C4orf52 (TMAB-03449) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
4. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (TMAB-03449) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (TMAB-03449) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (TMAB-03449) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human C4orf52

Human

SMIM20

Small integral membrane protein 20