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Anti-C1QL1 Polyclonal Antibody is a Rabbit antibody targeting C1QL1. Anti-C1QL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-C1QL1 Polyclonal Antibody is a Rabbit antibody targeting C1QL1. Anti-C1QL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Cow) |
Verified Activity | 1. Tissue/cell: mouse cerebel tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-C1QL1 Polyclonal Antibody, Unconjugated (TMAB-00260) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C1QL1) Polyclonal Antibody, Unconjugated (TMAB-00260) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-C1QL1 (TMAB-00260) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 32 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | C1q is part of the C1 enzyme complex, which activates the serum complement system. The residues of the globular domain in C1q share homology with several other secreted and membrane-bound collagen or collagen-like proteins, including pre-cerebellin and collagen types VIII and X, as well as the human and mouse genes encoding Apm1/BPB80 and AdipoQ/ACRP30, respectively. These various C1q-related proteins are found in adipose serum, corneal endothelium, chondrocytes and cerebral Purkinje cells. C1qL1 (complement component 1, q subcomponent-like 1), also known as CRF or C1QRF, is a polypeptide with a hydrophobic signal sequence, a collagenous region and a globular domain at the carboxy terminus, which shares homology to the C1q globular domain. C1qL1 transcripts are most abundant in areas of the nervous system that are associated with motor function, including cerebral Purkinje cells, the pons, the accessory olivary nucleus, and the red nucleus. The similarity of mouse C1qL1 to human C1qL1 suggests a conserved and important role for the protein. In humans, the gene encoding C1qL1 maps to chromosome 17q21. |
Immunogen | KLH conjugated synthetic peptide: human C1QL1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Complement,ECM Proteins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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