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Anti-C1QL1 Polyclonal Antibody

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Catalog No. TMAB-00260

Anti-C1QL1 Polyclonal Antibody is a Rabbit antibody targeting C1QL1. Anti-C1QL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-C1QL1 Polyclonal Antibody

Anti-C1QL1 Polyclonal Antibody

😃Good
Catalog No. TMAB-00260
Anti-C1QL1 Polyclonal Antibody is a Rabbit antibody targeting C1QL1. Anti-C1QL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$374 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-C1QL1 Polyclonal Antibody is a Rabbit antibody targeting C1QL1. Anti-C1QL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow)
Verified Activity
1. Tissue/cell: mouse cerebel tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-C1QL1 Polyclonal Antibody, Unconjugated (TMAB-00260) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C1QL1) Polyclonal Antibody, Unconjugated (TMAB-00260) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-C1QL1 (TMAB-00260) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 32 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundC1q is part of the C1 enzyme complex, which activates the serum complement system. The residues of the globular domain in C1q share homology with several other secreted and membrane-bound collagen or collagen-like proteins, including pre-cerebellin and collagen types VIII and X, as well as the human and mouse genes encoding Apm1/BPB80 and AdipoQ/ACRP30, respectively. These various C1q-related proteins are found in adipose serum, corneal endothelium, chondrocytes and cerebral Purkinje cells. C1qL1 (complement component 1, q subcomponent-like 1), also known as CRF or C1QRF, is a polypeptide with a hydrophobic signal sequence, a collagenous region and a globular domain at the carboxy terminus, which shares homology to the C1q globular domain. C1qL1 transcripts are most abundant in areas of the nervous system that are associated with motor function, including cerebral Purkinje cells, the pons, the accessory olivary nucleus, and the red nucleus. The similarity of mouse C1qL1 to human C1qL1 suggests a conserved and important role for the protein. In humans, the gene encoding C1qL1 maps to chromosome 17q21.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human C1QL1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Complement,ECM Proteins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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