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Anti-BUB3 Antibody (9N253)
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Catalog No. TMAC-00488
Alias WD repeat type I transmembrane protein A72.5, OTTHUMP00000020680, OTTHUMP00000020679, Mitotic checkpoint protein BUB3, Mitotic checkpoint component, Hbub3, C78067, Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of, Budding uninhibited by benzimidazoles 3 homolog (yeast), Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae), Budding uninhibited by benzimidazoles 3 homolog, Budding uninhibited by benzimidazoles 3, budding uninhibited by benomyl, BUB3L, BUB3_HUMAN, BUB3 mitotic checkpoint protein, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, bub3, BUB 3L, BUB 3, AW146323, AU043350, AU021329, AU019800, Aa2-050
Anti-BUB3 Antibody (9N253) is a Rabbit antibody targeting BUB3. Anti-BUB3 Antibody (9N253) can be used in WB,ICC,IF,IP,IHC,FCM.
Anti-BUB3 Antibody (9N253)
Anti-BUB3 Antibody (9N253)
😃Good
Catalog No. TMAC-00488Alias WD repeat type I transmembrane protein A72.5, OTTHUMP00000020680, OTTHUMP00000020679, Mitotic checkpoint protein BUB3, Mitotic checkpoint component, Hbub3, C78067, Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of, Budding uninhibited by benzimidazoles 3 homolog (yeast), Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae), Budding uninhibited by benzimidazoles 3 homolog, Budding uninhibited by benzimidazoles 3, budding uninhibited by benomyl, BUB3L, BUB3_HUMAN, BUB3 mitotic checkpoint protein, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, bub3, BUB 3L, BUB 3, AW146323, AU043350, AU021329, AU019800, Aa2-050
Anti-BUB3 Antibody (9N253) is a Rabbit antibody targeting BUB3. Anti-BUB3 Antibody (9N253) can be used in WB,ICC,IF,IP,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-BUB3 Antibody (9N253) is a Rabbit antibody targeting BUB3. Anti-BUB3 Antibody (9N253) can be used in WB,ICC,IF,IP,IHC,FCM. |
| Synonyms | WD repeat type I transmembrane protein A72.5, OTTHUMP00000020680, OTTHUMP00000020679, Mitotic checkpoint protein BUB3, Mitotic checkpoint component, Hbub3, C78067, Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of, Budding uninhibited by benzimidazoles 3 homolog (yeast), Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae), Budding uninhibited by benzimidazoles 3 homolog, Budding uninhibited by benzimidazoles 3, budding uninhibited by benomyl, BUB3L, BUB3_HUMAN, BUB3 mitotic checkpoint protein, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, bub3, BUB 3L, BUB 3, AW146323, AU043350, AU021329, AU019800, Aa2-050 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution. Positive control: Lane 1: A549, Lane 2: HL-60, Lane 3: A431, Lane 4: Rat colon. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Bub3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Bub3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bub3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse fallopian tissue using anti-Bub3 antibody. Counter stained with hematoxylin. 6. ICC staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Bub3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Bub3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of PC-3M cells with Bub3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: N-terminus human Bub3 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 37 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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