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Anti-BNIP3 Antibody (3L227)
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Catalog No. TMAC-00461
Alias Nip3, Bnip3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Anti-BNIP3 Antibody (3L227) is a Rabbit antibody targeting BNIP3. Anti-BNIP3 Antibody (3L227) can be used in WB,ICC/IF,IHC.
Anti-BNIP3 Antibody (3L227)
Anti-BNIP3 Antibody (3L227)
😃Good
Catalog No. TMAC-00461Alias Nip3, Bnip3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Anti-BNIP3 Antibody (3L227) is a Rabbit antibody targeting BNIP3. Anti-BNIP3 Antibody (3L227) can be used in WB,ICC/IF,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-BNIP3 Antibody (3L227) is a Rabbit antibody targeting BNIP3. Anti-BNIP3 Antibody (3L227) can be used in WB,ICC/IF,IHC. |
| Synonyms | Nip3, Bnip3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of BNIP3 on different cells lysates using anti- BNIP3 antibody at 1/500 dilution. Positive control: Line 1: Human skeletal, muscle Line 2: Mouse kidney. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BNIP3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BNIP3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BNIP3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BNIP3 antibody. Counter stained with hematoxylin. 6. ICC staining BNIP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining BNIP3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining BNIP3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins. BNIP-3 preferentially binds to Bcl-xL and induces apoptosis by suppressing the apoptosis activity of Bcl-xL. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 21/30 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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