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Anti-BMP-4 Polyclonal Antibody
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Catalog No. TMAB-00243
Anti-BMP-4 Polyclonal Antibody is a Rabbit antibody targeting BMP-4. Anti-BMP-4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-BMP-4 Polyclonal Antibody
Anti-BMP-4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00243
Anti-BMP-4 Polyclonal Antibody is a Rabbit antibody targeting BMP-4. Anti-BMP-4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Mouse,Sheep (predicted:Human,Rat) |
| Ig Type | IgG |
| Reactivity | Mouse,Sheep (predicted:Human,Rat) |
| Verified Activity | 1. Sample: Sheep skin (Sheep) Lysate at 40 μg Primary: Anti-BMP4 (TMAB-00243) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/45 kDa Observed band size: 50 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BMP4) Polyclonal Antibody, Unconjugated (TMAB-00243) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix. |
| Immunogen | KLH conjugated synthetic peptide: human BMP4 |
| Antigen Species | Human |
| Gene ID | 652 |
| Uniprot ID | |
| Biology Area | TGF,TGF,Kidney development,Gut specification,Nervous system development,TGF,Secreted |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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