Antibody Type: Recombinant Rabbit Monoclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

Reactivity: Human,Mouse (predicted:Rat)

IgG

6E793

Human,Mouse (predicted:Rat)

1. Cell line: SH-SY5Y
Fixation: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary dilution: 1:100
Secondary: Goat Anti-Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for TMAB-00239
2. Tissue: Mouse pancreas
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00239
3. Tissue: Mouse hippocampus
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:1000
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00239
4. Western blot analysis of BMAL1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (TMAB-00239, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: Rat brain tissue lysate
Lane 4: Mouse spleen tissue lysate
5. ICC staining of BMAL1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (TMAB-00239, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
6. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:2000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: PC-3
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 68 kDa
Observed MW: 75 kDa

WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:100

Monoclonal

KLH conjugated synthetic peptide: human BMAL1

Human

406

Blood Pressure regulation,Cardiac metabolism,Cholesterol Metabolism,HLH,Transcription Factors,Cholesterol Metabolism,Metabolism