Anti-Beclin-1 Polyclonal Antibody 2 is a Rabbit antibody targeting Beclin-1. Anti-Beclin-1 Polyclonal Antibody 2 can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Human,Mouse,Rat (predicted:Rabbit)

1. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Small intestine (Mouse) Lysate at 40 μg
Lane 3: Lung (Mouse) Lysate at 40 μg
Lane 4: Heart (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 6: Jurkat (Human) Cell Lysate at 30 μg
Lane 7: Raji (Human) Cell Lysate at 30 μg
Lane 8: Hela (Human) Cell Lysate at 30 μg
Lane 9: A431 (Human) Cell Lysate at 30 μg
Lane 10: HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-Beclin 1 (TMAB-00210) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55-60 kDa
Observed band size: 60 kDa
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IF=1:100-500; IHC-Fr=1:400-800; IHC-P=1:400-800; WB=1:500-1000

Polyclonal

KLH conjugated synthetic peptide: human BECN1

Human

BECN1

Beclin-1

Signal Transduction,Tumor suppressors,Autophagy,Tumor Suppressors,Autophagy and mitophagy,Host Immune Response

Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.