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Anti-Beclin-1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00210
Anti-Beclin-1 Polyclonal Antibody 2 is a Rabbit antibody targeting Beclin-1. Anti-Beclin-1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA.
Anti-Beclin-1 Polyclonal Antibody 2
Anti-Beclin-1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00210
Anti-Beclin-1 Polyclonal Antibody 2 is a Rabbit antibody targeting Beclin-1. Anti-Beclin-1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM,ELISA Reactivity: Human,Mouse,Rat (predicted:Pig,Bovine) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Bovine) |
| Verified Activity | 1. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Small intestine (Mouse) Lysate at 40 μg Lane 3: Lung (Mouse) Lysate at 40 μg Lane 4: Heart (Mouse) Lysate at 40 μg Lane 5: Cerebrum (Rat) Lysate at 40 μg Lane 6: Jurkat (Human) Cell Lysate at 30 μg Lane 7: Raji (Human) Cell Lysate at 30 μg Lane 8: Hela (Human) Cell Lysate at 30 μg Lane 9: A431 (Human) Cell Lysate at 30 μg Lane 10: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-Beclin 1 (TMAB-00210) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55-60 kDa Observed band size: 60 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (TMAB-00210) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM,ELISA |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500; FCM: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. |
| Immunogen | KLH conjugated synthetic peptide: human BECN1 |
| Antigen Species | Human |
| Gene ID | 8678 |
| Uniprot ID | |
| Biology Area | Signal Transduction,Tumor suppressors,Autophagy,Tumor Suppressors,Autophagy and mitophagy,Host Immune Response |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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