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Anti-BAX Polyclonal Antibody 4 is a Rabbit antibody targeting BAX. Anti-BAX Polyclonal Antibody 4 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM Reactivity: Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Sheep) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Sheep) |
Verified Activity | 1. Blank control (blue line): Hela (fixed with 80% methanol (5 min at-20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Bax antibody (TMAB-00191), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. 2. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-Bax (TMAB-00191) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 23 kDa |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg /test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential). |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
Immunogen | KLH conjugated synthetic peptide: human Bax |
Antigen Species | Human |
Gene ID | 581 |
Uniprot ID | |
Biology Area | Bcl 2 family,Metabolism,Bcl 2 family,Associated Proteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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