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Anti-BAX Antibody (7Y415)
😃Good
Catalog No. TMAB-00185
Alias Bcl-2-like protein 4, Bcl2-L-4, BCL2L4, Apoptosis regulator BAX
Anti-BAX Antibody (7Y415) is a Mouse antibody targeting BAX. Anti-BAX Antibody (7Y415) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-BAX Antibody (7Y415)
Anti-BAX Antibody (7Y415)
😃Good
Catalog No. TMAB-00185Alias Bcl-2-like protein 4, Bcl2-L-4, BCL2L4, Apoptosis regulator BAX
Anti-BAX Antibody (7Y415) is a Mouse antibody targeting BAX. Anti-BAX Antibody (7Y415) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-BAX Antibody (7Y415) is a Mouse antibody targeting BAX. Anti-BAX Antibody (7Y415) can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Bcl-2-like protein 4, Bcl2-L-4, BCL2L4, Apoptosis regulator BAX |
| Ig Type | IgG1 |
| Clone | 7Y415 |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (TMAB-00185) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nucleus staining. 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Bax (TMAB-00185) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa 3. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (TMAB-00185) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:1000-1500; IHC-P: 1:100-500; IHC-Fr: 1:200-500; IF: 1:200-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential). |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Bax |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Bcl 2 family,Metabolism,Bcl 2 family,Associated Proteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis |
| Molecular Weight | Theoretical: 21 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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