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Anti-BAX Antibody (3I323)
😃Good
Catalog No. TMAB-00186
Alias Bcl-2-like protein 4, Bcl2-L-4, BCL2L4, Apoptosis regulator BAX
Anti-BAX Antibody (3I323) is a Mouse antibody targeting BAX. Anti-BAX Antibody (3I323) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-BAX Antibody (3I323)
Anti-BAX Antibody (3I323)
😃Good
Catalog No. TMAB-00186Alias Bcl-2-like protein 4, Bcl2-L-4, BCL2L4, Apoptosis regulator BAX
Anti-BAX Antibody (3I323) is a Mouse antibody targeting BAX. Anti-BAX Antibody (3I323) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-BAX Antibody (3I323) is a Mouse antibody targeting BAX. Anti-BAX Antibody (3I323) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | Bcl-2-like protein 4, Bcl2-L-4, BCL2L4, Apoptosis regulator BAX |
| Ig Type | IgG1 |
| Clone | 3I323 |
| Reactivity | Human (predicted:Monkey,Insect/Yeast) |
| Verified Activity | 1. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (TMAB-00186) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Sample: Lane 1: Human 293T cell lysates Lane 2: Human HT-1080 cell lysates Lane 3: Human Raji cell lysates Lane 4: Human SH-SY5Y cell lysates Primary: Anti-Bax (TMAB-00186) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 20 kDa |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:200-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. Isoform Beta: Cytoplasm. Isoform Gamma: Cytoplasm. Isoform Delta: Cytoplasm (Potential). |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Bax |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Bcl 2 family,Metabolism,Bcl 2 family,Associated Proteins,Bcl2 Family,Apoptosis marker and proteins ELISA kits,Apoptosis |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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