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Anti-Axin-2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00176
Anti-Axin-2 Polyclonal Antibody is a Rabbit antibody targeting Axin-2. Anti-Axin-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Axin-2 Polyclonal Antibody
Anti-Axin-2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00176
Anti-Axin-2 Polyclonal Antibody is a Rabbit antibody targeting Axin-2. Anti-Axin-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Mouse,Rat (predicted:Human,Dog,Horse) |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Axin 2) Polyclonal Antibody, Unconjugated (TMAB-00176) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Axin 2) Polyclonal Antibody, Unconjugated (TMAB-00176) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. 3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Axin 2 Polyclonal Antibody, Unconjugated (TMAB-00176) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Axin 2 Polyclonal Antibody, Unconjugated (TMAB-00176) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Axin 2 (TMAB-00176) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 93 kDa Observed band size: 93 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B. Involvement in disease:Defects in AXIN2 are involved in colorectal cancer (CRC). They appear to be specifically associated with defective mismatch repair. Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS). Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. |
| Immunogen | KLH conjugated synthetic peptide: human Axin 2 corresponding |
| Antigen Species | Human |
| Gene ID | 8313 |
| Uniprot ID | |
| Biology Area | Cell differentiation,Cell differentiation,Proto-oncogenes,Neurogenesis,Cytoplasmic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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