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Anti-ATP5A1 Antibody (7X811)

Catalog No. TMAC-00339 Copy Product Info
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Anti-ATP5A1 Antibody (7X811) is a Rabbit antibody targeting ATP5A1. Anti-ATP5A1 Antibody (7X811) can be used in FCM,ICC/IF,IHC,WB.

Anti-ATP5A1 Antibody (7X811)

Copy Product Info
🥰Excellent
Catalog No. TMAC-00339

Anti-ATP5A1 Antibody (7X811) is a Rabbit antibody targeting ATP5A1. Anti-ATP5A1 Antibody (7X811) can be used in FCM,ICC/IF,IHC,WB.

Anti-ATP5A1 Antibody
(7X811)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2967-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Appearance:Liquid
Color:Transparent
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Product Introduction

Bioactivity
Description
Anti-ATP5A1 Antibody (7X811) is a Rabbit antibody targeting ATP5A1. Anti-ATP5A1 Antibody (7X811) can be used in FCM,ICC/IF,IHC,WB.
Ig Type
IgG
Clone
7X811
Reactivity
Human,Mouse,Rat
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.
4. ICC staining ATP5A1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining ATP5A1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining ATP5A1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Hela cells with ATP5A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
8. All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
9. Western blot analysis of ATP5A1 expression in HepG2 cell lysate.
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Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200; FCM: 1:20-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Research BackgroundMitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three α chains and three β chains, which are weakly homologous, as well as one γ chain, one δ chain and one e chain. F0 consists of three subunits: a, b and c. The α chain of F1 is a regulatory subunit that contains 509 amino acids. Mitochondrial ATPase α chain (ATP5A) localizes to the mitochondria and catalyzes ATP synthesis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthesized peptide: human ATP5A
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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