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Anti-ATF-5 Antibody (6Y194) is a Rabbit antibody targeting ATF-5. Anti-ATF-5 Antibody (6Y194) can be used in ICC/IF,IHC,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
| Description | Anti-ATF-5 Antibody (6Y194) is a Rabbit antibody targeting ATF-5. Anti-ATF-5 Antibody (6Y194) can be used in ICC/IF,IHC,IP,WB. |
| Ig Type | IgG |
| Clone | 6Y194 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: PC-12. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin. 6. ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 31 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |

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