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Anti-ART4/CD297 Antibody (8Y723) is a Mouse antibody targeting ART4/CD297. Anti-ART4/CD297 Antibody (8Y723) can be used in IHC-P.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $198 | 7-10 days |
Description | Anti-ART4/CD297 Antibody (8Y723) is a Mouse antibody targeting ART4/CD297. Anti-ART4/CD297 Antibody (8Y723) can be used in IHC-P. |
Alias | ADP-ribosyltransferase 4 (Dombrock blood group) |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 8Y723 |
Reactivity | Rat |
Verified Activity | 1. Immunochemical staining of rat ART4 in rat kidney with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of rat ART4 in rat liver with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). |
Application | IHC-P |
Recommended Dose | IHC-P: 1:50-1:200 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD297 (rR CD297; TMPY-03312; NP_001166980.1; Met1-Lys269). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. |
Conjucates | Unconjugated |
Immunogen | Recombinant Rat CD297 Protein (TMPY-03312) |
Antigen Species | Rat |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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