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Anti-Ano1 Antibody (1J659)
😃Good
Catalog No. TMAY-00516
Alias TMEM16A, TAOS2, ORAOV2, MYMY7, INDMS, FLJ10261, DOG1, ANO1
Anti-Ano1 Antibody (1J659) is a Rabbit antibody targeting Ano1. Anti-Ano1 Antibody (1J659) can be used in WB.
Anti-Ano1 Antibody (1J659)
Anti-Ano1 Antibody (1J659)
😃Good
Catalog No. TMAY-00516Alias TMEM16A, TAOS2, ORAOV2, MYMY7, INDMS, FLJ10261, DOG1, ANO1
Anti-Ano1 Antibody (1J659) is a Rabbit antibody targeting Ano1. Anti-Ano1 Antibody (1J659) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB Reactivity: Human |
| Alias | TMEM16A, TAOS2, ORAOV2, MYMY7, INDMS, FLJ10261, DOG1, ANO1 |
| Ig Type | Rabbit IgG |
| Clone | 1J659 |
| Reactivity | Human |
| Verified Activity | Anti-ANO1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:114 kDa. -Observed band size:114 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the C-terminus of the Human Ano1. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Anoctamin1 (Ano1 and TMEM16A) is a calcium-activated chloride channel specifically expressed in the interstitial cells of Cajal (ICC) of the gastrointestinal tract muscularis propria. Ano1 is a recently discovered Ca(2+)-activated Cl(-) channel expressed on interstitial cells of Cajal (ICC) that has been implicated in slow-wave activity in the gut. However, Ano1 is expressed on all classes of ICC, even those that do not contribute to generation of the slow wave, suggesting that Ano1 may have an alternate function in these cells. Ano1 is also highly expressed in gastrointestinal stromal tumors. Mice lacking Ano1 had fewer proliferating ICC in whole mount preparations and in culture, raising the possibility that Ano1 is involved in proliferation. Ano1 had less phosphorylated retinoblastoma protein compared with controls. Ano1 regulates proliferation at the G(1)/S transition of the cell cycle and may play a role in tumorigenesis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: C-terminus of the Human Ano1 |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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