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Anti-Androgen receptor Antibody (3T582)
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Catalog No. TMAC-00200
Alias Nuclear receptor subfamily 3 group C member 4, NR3C4, Dihydrotestosterone receptor, DHTR, AR, Androgen receptor
Anti-Androgen receptor Antibody (3T582) is a Rabbit antibody targeting Androgen receptor. Anti-Androgen receptor Antibody (3T582) can be used in WB,ICC/IF,IHC,FCM.
Anti-Androgen receptor Antibody (3T582)
Anti-Androgen receptor Antibody (3T582)
😃Good
Catalog No. TMAC-00200Alias Nuclear receptor subfamily 3 group C member 4, NR3C4, Dihydrotestosterone receptor, DHTR, AR, Androgen receptor
Anti-Androgen receptor Antibody (3T582) is a Rabbit antibody targeting Androgen receptor. Anti-Androgen receptor Antibody (3T582) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-Androgen receptor Antibody (3T582) is a Rabbit antibody targeting Androgen receptor. Anti-Androgen receptor Antibody (3T582) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Nuclear receptor subfamily 3 group C member 4, NR3C4, Dihydrotestosterone receptor, DHTR, AR, Androgen receptor |
| Ig Type | IgG |
| Clone | 3T582 |
| Reactivity | Human |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin. 3. ICC staining Androgen receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Androgen receptor in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Androgen receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of MCF-7 cells with Androgen receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and male reproductive physiology. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind the two biologically active androgens, testosterone (T) and dihydrotestosterone (DHT), with high and nearly identical affinities; however, the rates of association and dissociation of T are about three times more rapid than those of DHT. This difference has resulted in speculation as to whether these differences in binding kinetics could account for the different physiological effects of T and DHT. A striking feature of AR is its rapid degradation in the absence of ligand. It is now well established that androgen binding results in an at least six-fold increase in androgen stability and that ligand-induced stabilization of AR is highly androgen- specific. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 99 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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