Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep)

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PRKAA2/AMPK alpha 2 Polyclonal Antibody, Unconjugated (TMAB-00116) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PRKAA2/AMPK alpha 2 Polyclonal Antibody, Unconjugated (TMAB-00116) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane1: Brain (Rat) Lysate at 30 μg
Lane2: Heart (Rat) Lysate at 30 μg
Primary: Anti-PRKAA2/AMPK alpha 2 (TMAB-00116) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 62 kDa
Observed band size: 62 kDa
4. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: Jurkat (Human) Cell Lysate at 30 μg
Lane 3: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-AMPK alpha 2 (TMAB-00116) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human AMPK alpha 2

Human

5563

Integration of energy metabolism,Response to hypoxia,Metabolism,Integration of energy,Fatty acids,Hypoxia,Fatty acid oxidation,Metabolism,Other Kinases