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Anti-AMPK alpha 2 Polyclonal Antibody
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Catalog No. TMAB-00116
Alias Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA2, PRKAA, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, AMPKα2, AMPKα 2, AMPKalpha2, AMPKa2, AMPK2, AMPK α2, AMPK α 2, AMPK subunit alpha-2, AMPK alpha 2 chain, AMPK a2, AMPK 2, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2, 5'-AMP-activated protein kinase catalytic subunit alpha-2
Anti-AMPK alpha 2 Polyclonal Antibody is a Rabbit antibody targeting AMPK alpha 2. Anti-AMPK alpha 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AMPK alpha 2 Polyclonal Antibody
Anti-AMPK alpha 2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00116Alias Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA2, PRKAA, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, AMPKα2, AMPKα 2, AMPKalpha2, AMPKa2, AMPK2, AMPK α2, AMPK α 2, AMPK subunit alpha-2, AMPK alpha 2 chain, AMPK a2, AMPK 2, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2, 5'-AMP-activated protein kinase catalytic subunit alpha-2
Anti-AMPK alpha 2 Polyclonal Antibody is a Rabbit antibody targeting AMPK alpha 2. Anti-AMPK alpha 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-AMPK alpha 2 Polyclonal Antibody is a Rabbit antibody targeting AMPK alpha 2. Anti-AMPK alpha 2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA2, PRKAA, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, AMPKα2, AMPKα 2, AMPKalpha2, AMPKa2, AMPK2, AMPK α2, AMPK α 2, AMPK subunit alpha-2, AMPK alpha 2 chain, AMPK a2, AMPK 2, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2, 5'-AMP-activated protein kinase catalytic subunit alpha-2 |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PRKAA2/AMPK alpha 2 Polyclonal Antibody, Unconjugated (TMAB-00116) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PRKAA2/AMPK alpha 2 Polyclonal Antibody, Unconjugated (TMAB-00116) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane1: Brain (Rat) Lysate at 30 μg Lane2: Heart (Rat) Lysate at 30 μg Primary: Anti-PRKAA2/AMPK alpha 2 (TMAB-00116) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution; Predicted band size: 62 kDa Observed band size: 62 kDa 4. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: Jurkat (Human) Cell Lysate at 30 μg Lane 3: 293T (Human) Cell Lysate at 30 μg Primary: Anti-AMPK alpha 2 (TMAB-00116) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 60 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human AMPK alpha 2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Integration of energy metabolism,Response to hypoxia,Metabolism,Integration of energy,Fatty acids,Hypoxia,Fatty acid oxidation,Metabolism,Other Kinases |
| Molecular Weight | Theoretical: 62 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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