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Anti-AIM2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00083
Alias PYHIN4, absent in melanoma 2

Anti-AIM2 Polyclonal Antibody is a Rabbit antibody targeting AIM2. Anti-AIM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-AIM2 Polyclonal Antibody

Anti-AIM2 Polyclonal Antibody

😃Good
Catalog No. TMAB-00083Alias PYHIN4, absent in melanoma 2
Anti-AIM2 Polyclonal Antibody is a Rabbit antibody targeting AIM2. Anti-AIM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-AIM2 Polyclonal Antibody is a Rabbit antibody targeting AIM2. Anti-AIM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasPYHIN4, absent in melanoma 2
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-AIM2 Polyclonal Antibody, Unconjugated (TMAB-00083) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AIM2) Polyclonal Antibody, Unconjugated (TMAB-00083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AIM2) Polyclonal Antibody, Unconjugated (TMAB-00083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AIM2) Polyclonal Antibody, Unconjugated (TMAB-00083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human A549 cell lysates
Lane 3: Human U87MG cell lysates
Lane 4: Human A431 cell lysates
Primary: Anti-AIM2 (TMAB-00083) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 46 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus (By similarity). Cytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundAIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse AIM2.
Antigen Species
Mouse
Gene ID
Uniprot ID
Biology Area
Tumor Suppressors,Tumor suppressors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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