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Anti-AIM2 Polyclonal Antibody is a Rabbit antibody targeting AIM2. Anti-AIM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-AIM2 Polyclonal Antibody is a Rabbit antibody targeting AIM2. Anti-AIM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | PYHIN4, absent in melanoma 2 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-AIM2 Polyclonal Antibody, Unconjugated (TMAB-00083) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AIM2) Polyclonal Antibody, Unconjugated (TMAB-00083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AIM2) Polyclonal Antibody, Unconjugated (TMAB-00083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AIM2) Polyclonal Antibody, Unconjugated (TMAB-00083) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human A549 cell lysates Lane 3: Human U87MG cell lysates Lane 4: Human A431 cell lysates Primary: Anti-AIM2 (TMAB-00083) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 46 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus (By similarity). Cytoplasm. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. |
Immunogen | KLH conjugated synthetic peptide: mouse AIM2. |
Antigen Species | Mouse |
Gene ID | |
Uniprot ID | |
Biology Area | Tumor Suppressors,Tumor suppressors |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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