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Anti-AGTR1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00079
Anti-AGTR1 Polyclonal Antibody 2 is a Rabbit antibody targeting AGTR1. Anti-AGTR1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AGTR1 Polyclonal Antibody 2
Anti-AGTR1 Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00079
Anti-AGTR1 Polyclonal Antibody 2 is a Rabbit antibody targeting AGTR1. Anti-AGTR1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (TMAB-00079) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Liver (Mouse) Lysate at 40 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Placenta (Mouse) Lysate at 40 μg Lane 4: Kidney (Mouse) Lysate at 40 μg Lane 5: Embryo (Mouse) Lysate at 40 μg Lane 6: Cerebrum (Mouse) Lysate at 40 μg Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 μg Lane 8: Liver (Rat) Lysate at 40 μg Lane 9: Heart (Rat) Lysate at 40 μg Lane 10: Kidney (Rat) Lysate at 40 μg Lane 11: Cerebrum (Rat) Lysate at 40 μg Lane 12: Embryo (Rat) Lysate at 40 μg Primary: Anti-Angiotensin II type 1A receptor (TMAB-00079) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 46 kDa 3. Sample: A549 (Human) Cell Lysate at 430 μg Primary: Anti-Angiotensin II type 1A receptor (TMAB-00079) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 41/51 kDa 4. Sample: Liver (Mouse) Lysate at 40 μg Lung (Mouse) Lysate at 40 μg Adrenal gland (Rat) Lysate at 40 μg Primary: Anti-Angiotensin II type 1A receptor (TMAB-00079) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 56 kDa 5. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (TMAB-00079) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least five transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human Angiotensin II type 1A receptor |
| Antigen Species | Human |
| Gene ID | 185 |
| Uniprot ID | |
| Biology Area | Cellular metabolic process,Angiogenic growth factors,Blood Pressure regulation,Cancer |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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