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Anti-AGR2 Antibody (7E227)

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Catalog No. TMAC-00099
Alias Secreted Cement Gland Protein XAG-2 Homolog, HPC8, hAG-2, Anterior Gradient Protein 2 Homolog, AGR2, AG-2, AG2

Anti-AGR2 Antibody (7E227) is a Rabbit antibody targeting AGR2. Anti-AGR2 Antibody (7E227) can be used in WB,ICC,IHC,IP,FCM.

Anti-AGR2 Antibody (7E227)

Anti-AGR2 Antibody (7E227)

😃Good
Catalog No. TMAC-00099Alias Secreted Cement Gland Protein XAG-2 Homolog, HPC8, hAG-2, Anterior Gradient Protein 2 Homolog, AGR2, AG-2, AG2
Anti-AGR2 Antibody (7E227) is a Rabbit antibody targeting AGR2. Anti-AGR2 Antibody (7E227) can be used in WB,ICC,IHC,IP,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-AGR2 Antibody (7E227) is a Rabbit antibody targeting AGR2. Anti-AGR2 Antibody (7E227) can be used in WB,ICC,IHC,IP,FCM.
SynonymsSecreted Cement Gland Protein XAG-2 Homolog, HPC8, hAG-2, Anterior Gradient Protein 2 Homolog, AGR2, AG-2, AG2
Ig Type
IgG
Clone
7E227
Reactivity
Human
Verified Activity
1. Western blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AGR2 antibody. Counter stained with hematoxylin.
3. ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining AGR2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining AGR2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of Hela cells with AGR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundAGR2 (Anterior Gradient Protein 2), also known as AG2, GOB-4 or HAG-2, is a member of the anterior gradient homolog family. It is the human ortholog of XAG-2, the secreted Xenopus laevis Anterior Gradient protein. In X. laevis, it is involved in cement gland differentiation and neural marker gene expression. AGR2 is a secretory protein encoded by two different AGR2 transcripts. It interacts with LYPD3 and α-dystroglycan (DAG-1). AGR2 is ubiquitously expressed with up-regulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR). AGR2 may serve as a potential therapeutic marker for various cancers.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 17 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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