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Anti-ADRB3 Polyclonal Antibody
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Catalog No. TMAB-00072
Anti-ADRB3 Polyclonal Antibody is a Rabbit antibody targeting ADRB3. Anti-ADRB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ADRB3 Polyclonal Antibody
Anti-ADRB3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00072
Anti-ADRB3 Polyclonal Antibody is a Rabbit antibody targeting ADRB3. Anti-ADRB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Sheep) |
| Verified Activity | 1. Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (TMAB-00072) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: K562 (Human) Cell Lysate at 40 μg Primary: Anti-beta 3 Adrenergic Receptor (TMAB-00072) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 54 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein. |
| Immunogen | KLH conjugated synthetic peptide: human ADRB3 |
| Antigen Species | Human |
| Gene ID | 155 |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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