Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Dog,Pig)

IgG

Human,Mouse,Rat (predicted:Dog,Pig)

1. Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated (TMAB-00068) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: skin of rat foot; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated (TMAB-00068) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Heart (Mouse) Lysate at 30 μg
Heart (Rat) Lysate at 30 μg
Primary: Anti-ADRB1 (TMAB-00068) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/3000 dilution
Predicted band size: 52 kDa
Observed band size: 55 kDa
4. Tissue/cell: mouse kidney cell;
Incubation:
Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated (TMAB-00068-FITC) 1:200, 40 minutes at 37°C.
Green line: Isotype Control ( rabbit IgG/FITC) 1:200, 40 minutes at 37°C.
5. Blank control: U937. Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (TMAB-00068)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
6. Blank control: U937. Primary Antibody (green line): Rabbit Anti-antibody (TMAB-00068)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human ADRB1

Human

153

GPCR,Adrenalin,Adrenergic Receptors,Blood Pressure regulation