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Anti-Adenylosuccinate Lyase Antibody (9U559)
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Catalog No. TMAY-01827
Alias ASL, ASASE, AMPS, adenylosuccinate lyase
Anti-Adenylosuccinate Lyase Antibody (9U559) is a Rabbit antibody targeting Adenylosuccinate Lyase. Anti-Adenylosuccinate Lyase Antibody (9U559) can be used in WB,ICC/IF,IP.
Anti-Adenylosuccinate Lyase Antibody (9U559)
Anti-Adenylosuccinate Lyase Antibody (9U559)
😃Good
Catalog No. TMAY-01827Alias ASL, ASASE, AMPS, adenylosuccinate lyase
Anti-Adenylosuccinate Lyase Antibody (9U559) is a Rabbit antibody targeting Adenylosuccinate Lyase. Anti-Adenylosuccinate Lyase Antibody (9U559) can be used in WB,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ICC/IF, IP Reactivity: Human |
| Alias | ASL, ASASE, AMPS, adenylosuccinate lyase |
| Ig Type | Rabbit IgG |
| Clone | 9U559 |
| Reactivity | Human |
| Verified Activity | 1. Anti-ADSL rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: Hela Whole Cell Lysate. -Lane D: C6 Whole Cell Lysate. -Lane E: Raw264.7 Whole Cell Lysate. -Lane F: NIH-3T3 Whole Cell Lysate. -Lane G: HCT-116 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:55 kDa. -Observed band size:55 kDa. 2. Immunofluorescence staining of ADSL in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ADSL monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). 3. ADSL was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-ADSL rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ADSL rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 50 kDa. -Observed band size: 50 kDa |
| Application | WB,ICC/IF,IP |
| Recommended Dose | WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ADSL / Adenylosuccinate Lyase (rh ADSL / Adenylosuccinate Lyase; TMPY-02552; P30566-1; Met1-Leu484). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ADSL / Adenylosuccinate Lyase protein (TMPY-02552) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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