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Anti-ACVR2B Polyclonal Antibody

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Catalog No. TMAB-00052
Alias HTX4, ActR-IIB, ACTRIIB, Activin RIIB, activin A receptor, type IIB

Anti-ACVR2B Polyclonal Antibody is a Rabbit antibody targeting ACVR2B. Anti-ACVR2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ACVR2B Polyclonal Antibody

Anti-ACVR2B Polyclonal Antibody

😃Good
Catalog No. TMAB-00052Alias HTX4, ActR-IIB, ACTRIIB, Activin RIIB, activin A receptor, type IIB
Anti-ACVR2B Polyclonal Antibody is a Rabbit antibody targeting ACVR2B. Anti-ACVR2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-ACVR2B Polyclonal Antibody is a Rabbit antibody targeting ACVR2B. Anti-ACVR2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasHTX4, ActR-IIB, ACTRIIB, Activin RIIB, activin A receptor, type IIB
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Activin Receptor Type IIB) Polyclonal Antibody, Unconjugated (TMAB-00052) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
K562 (Human) Cell Lysate at 30 μg
293t (Human) Cell Lysate at 30 μg
Primary: Anti-Activin Receptor Type IIB (TMAB-00052) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
3. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Kidney tissue lysates
Lane 5: Human K562 cell lysates
Lane 6: Human SH-SY5Y cell lysates
Lane 7: Human Jurkat cell lysates
Lane 8: Human MOLT4 cell lysates
Primary: Anti-Activin Receptor Type IIB (TMAB-00052) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundActivin receptor type-2B( Activin receptor type IIB; ACTR-IIB ) On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. [Subcellular Location] Membrane; Single-pass type I membrane protein. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ACVR2B/ACTR-IIB
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Actin Binding Proteins,Other Kinases,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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