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Anti-ACVR2B Polyclonal Antibody
😃Good
Catalog No. TMAB-00052
Alias HTX4, ActR-IIB, ACTRIIB, Activin RIIB, activin A receptor, type IIB
Anti-ACVR2B Polyclonal Antibody is a Rabbit antibody targeting ACVR2B. Anti-ACVR2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ACVR2B Polyclonal Antibody
Anti-ACVR2B Polyclonal Antibody
😃Good
Catalog No. TMAB-00052Alias HTX4, ActR-IIB, ACTRIIB, Activin RIIB, activin A receptor, type IIB
Anti-ACVR2B Polyclonal Antibody is a Rabbit antibody targeting ACVR2B. Anti-ACVR2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ACVR2B Polyclonal Antibody is a Rabbit antibody targeting ACVR2B. Anti-ACVR2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | HTX4, ActR-IIB, ACTRIIB, Activin RIIB, activin A receptor, type IIB |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Activin Receptor Type IIB) Polyclonal Antibody, Unconjugated (TMAB-00052) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: K562 (Human) Cell Lysate at 30 μg 293t (Human) Cell Lysate at 30 μg Primary: Anti-Activin Receptor Type IIB (TMAB-00052) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa 3. Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Mouse Muscle tissue lysates Lane 3: Mouse Testis tissue lysates Lane 4: Mouse Kidney tissue lysates Lane 5: Human K562 cell lysates Lane 6: Human SH-SY5Y cell lysates Lane 7: Human Jurkat cell lysates Lane 8: Human MOLT4 cell lysates Primary: Anti-Activin Receptor Type IIB (TMAB-00052) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 60 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Activin receptor type-2B( Activin receptor type IIB; ACTR-IIB ) On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. [Subcellular Location] Membrane; Single-pass type I membrane protein. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. |
| Immunogen | KLH conjugated synthetic peptide: human ACVR2B/ACTR-IIB |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Actin Binding Proteins,Other Kinases,Surface Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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