Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF

Reactivity: Human,Rat (predicted:Mouse,Cow,Goat)

IgG

Human,Rat (predicted:Mouse,Cow,Goat)

1. Paraformaldehyde-fixed, paraffin embedded (Human pituitary tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ACTH (1-39)) Polyclonal Antibody, Unconjugated (TMAB-00051) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ACTh (1-39) Polyclonal Antibody, Unconjugated (TMAB-00051) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ACTh (1-39) Polyclonal Antibody, Unconjugated (TMAB-00051) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.

IHC-P,IHC-Fr,IF

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ACTH

Human

5443

Hormone biosynthesis,Hormone biosynthesis,Hypothalamic pituitary adrenal axis,Hormones,Hormones