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Anti-ABCG2 Antibody (3U334)
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Catalog No. TMAB-02164
Alias UAQTL1, Placenta-specific ATP-binding cassette transporter, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance-associated protein, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG2_HUMAN, ABCG 2, ABC15
Anti-ABCG2 Antibody (3U334) is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Antibody (3U334) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ABCG2 Antibody (3U334)
Anti-ABCG2 Antibody (3U334)
Copy Product Info😃Good
Catalog No. TMAB-02164Alias UAQTL1, Placenta-specific ATP-binding cassette transporter, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance-associated protein, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG2_HUMAN, ABCG 2, ABC15
Anti-ABCG2 Antibody (3U334) is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Antibody (3U334) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ABCG2 Antibody (3U334) is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Antibody (3U334) can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | UAQTL1, Placenta-specific ATP-binding cassette transporter, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance-associated protein, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG2_HUMAN, ABCG 2, ABC15 |
| Ig Type | IgG |
| Clone | 3U334 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ABCG2 Monoclonal Antibody, Unconjugated (TMAB-02164) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ABCG2 Monoclonal Antibody, Unconjugated (TMAB-02164) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 3. 25 ug total protein per lane of various lysates (see on figure) probed with ABCG2 monoclonal antibody, unconjugated (TMAB-02164) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 4. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCG2 Monoclonal Antibody, Unconjugated (TMAB-02164) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei. 5. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ABCG2 Monoclonal Antibody, Unconjugated (TMAB-02164) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Tissue Specificity | Highly expressed in placenta. Low expression in small intestine, liver and colon. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone. |
| Immunogen | A synthesized peptide: human ABCG2 |
| Antigen Species | Human |
| Gene Name | ABCG2 |
| Gene ID | |
| Protein Name | Broad substrate specificity ATP-binding cassette transporter ABCG2 |
| Uniprot ID | |
| Function | Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. |
| Molecular Weight | Theoretical: 72 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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