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Anti-5T4 Antibody (6K317)
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Catalog No. TMAB-02115
Alias Trophoblast glycoprotein, TPBG_HUMAN, TPBG, M6P1, 5T4AG, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4
Anti-5T4 Antibody (6K317) is a Rabbit antibody targeting 5T4. Anti-5T4 Antibody (6K317) can be used in WB,IHC-P,IHC-Fr.
Anti-5T4 Antibody (6K317)
Anti-5T4 Antibody (6K317)
Copy Product Info😃Good
Catalog No. TMAB-02115Alias Trophoblast glycoprotein, TPBG_HUMAN, TPBG, M6P1, 5T4AG, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4
Anti-5T4 Antibody (6K317) is a Rabbit antibody targeting 5T4. Anti-5T4 Antibody (6K317) can be used in WB,IHC-P,IHC-Fr.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-5T4 Antibody (6K317) is a Rabbit antibody targeting 5T4. Anti-5T4 Antibody (6K317) can be used in WB,IHC-P,IHC-Fr. |
| Synonyms | Trophoblast glycoprotein, TPBG_HUMAN, TPBG, M6P1, 5T4AG, 5T4 oncotrophoblast glycoprotein, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 |
| Ig Type | IgG |
| Clone | 6K317 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5T4 Monoclonal Antibody, Unconjugated (TMAB-02115) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5T4 Monoclonal Antibody, Unconjugated (TMAB-02115) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. 25 μg total protein per lane of various lysates (see on figure) probed with 5T4 monoclonal antibody, unconjugated (TMAB-02115) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane. |
| Tissue Specificity | Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | TPBG is a 420 amino acid single-pass type I membrane protein expressed by all types of trophoblasts as early as 9 weeks of development. TPBG contains an N-terminal putative signal sequence, a 310-residue extracellular region, a membrane anchorage domain and a 44-amino acid cytoplasmic tail with a potential phosphorylation site. The extracellular region has seven potential N-glycosylation sites and seven leucine-rich repeats, which are located in two regions separated by a hydrophilic stretch. Suggested to be involved in cell adhesion, TPBG may also be associated with tumor growth and progression. |
| Immunogen | KLH conjugated synthetic peptide: human TPBG/5T4 |
| Antigen Species | Human |
| Gene Name | Tpbg |
| Gene ID | |
| Protein Name | Trophoblast glycoprotein |
| Uniprot ID |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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