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Anti-14-3-3 epsilon Polyclonal Antibody

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Catalog No. TMAB-02078
Alias YWHAE, tyrosine 3/tryptophan 5-monooxygenase activation protein epsilon polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide, protein kinase C inhibitor protein-1, Protein kinase C inhibitor protein1, mitochondrial import stimulation factor L subunit, MDS, MDCR, KCIP-1, KCIP1, KCIP 1, 1433E_HUMAN, 14-3-3E, 14-3-3 protein epsilon, 14-3-3 epsilon, 14-3-3 E, 14 3 3E, 14 3 3 epsilon, 14 3 3 E

Anti-14-3-3 epsilon Polyclonal Antibody is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-14-3-3 epsilon Polyclonal Antibody

Anti-14-3-3 epsilon Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-02078Alias YWHAE, tyrosine 3/tryptophan 5-monooxygenase activation protein epsilon polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide, protein kinase C inhibitor protein-1, Protein kinase C inhibitor protein1, mitochondrial import stimulation factor L subunit, MDS, MDCR, KCIP-1, KCIP1, KCIP 1, 1433E_HUMAN, 14-3-3E, 14-3-3 protein epsilon, 14-3-3 epsilon, 14-3-3 E, 14 3 3E, 14 3 3 epsilon, 14 3 3 E
Anti-14-3-3 epsilon Polyclonal Antibody is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-14-3-3 epsilon Polyclonal Antibody is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
SynonymsYWHAE, tyrosine 3/tryptophan 5-monooxygenase activation protein epsilon polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide, protein kinase C inhibitor protein-1, Protein kinase C inhibitor protein1, mitochondrial import stimulation factor L subunit, MDS, MDCR, KCIP-1, KCIP1, KCIP 1, 1433E_HUMAN, 14-3-3E, 14-3-3 protein epsilon, 14-3-3 epsilon, 14-3-3 E, 14 3 3E, 14 3 3 epsilon, 14 3 3 E
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Cow,Rabbit,Sheep)
Verified Activity
1. Sample:
A431 (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
NIH/3T3 (Mouse) Cell Lysate at 30 μg
Primary: Anti-14-3-3 epsilon (TMAB-02078) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (TMAB-02078) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (TMAB-02078) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Placenta tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Human 293T cell lysates
Lane 7: Human A375 cell lysates
Lane 8: Human SW480 cell lysates
Primary: Anti-14-3-3 epsilon (TMAB-02078) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human 14-3-3E
Antigen Species
Human
Gene Name
YWHAE
Gene ID
Protein Name
14-3-3 protein epsilon
Uniprot ID
Function
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Chemical Properties
Molecular WeightTheoretical: 29 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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