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Anti-14-3-3 epsilon Polyclonal Antibody (Synonyms: YWHAE, tyrosine 3/tryptophan 5-monooxygenase activation prot...)
Catalog No. TMAB-02078 Copy Product Info
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Anti-14-3-3 epsilon Polyclonal Antibody is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-14-3-3 epsilon Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-02078
Synonyms YWHAE, tyrosine 3/tryptophan 5-monooxygenase activation protein epsilon polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide, protein kinase C inhibitor protein-1, Protein kinase C inhibitor protein1, mitochondrial import stimulation factor L subunit, MDS, MDCR, KCIP-1, KCIP1, KCIP 1, 1433E_HUMAN, 14-3-3E, 14-3-3 protein epsilon, 14-3-3 epsilon, 14-3-3 E, 14 3 3E, 14 3 3 epsilon, 14 3 3 E
Anti-14-3-3 epsilon Polyclonal Antibody is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-14-3-3 epsilon Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
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| Description | Anti-14-3-3 epsilon Polyclonal Antibody is a Rabbit antibody targeting 14-3-3 epsilon. Anti-14-3-3 epsilon Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Synonyms | YWHAE, tyrosine 3/tryptophan 5-monooxygenase activation protein epsilon polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide, protein kinase C inhibitor protein-1, Protein kinase C inhibitor protein1, mitochondrial import stimulation factor L subunit, MDS, MDCR, KCIP-1, KCIP1, KCIP 1, 1433E_HUMAN, 14-3-3E, 14-3-3 protein epsilon, 14-3-3 epsilon, 14-3-3 E, 14 3 3E, 14 3 3 epsilon, 14 3 3 E |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Sample: A431 (Human) Cell Lysate at 30 μg A549 (Human) Cell Lysate at 30 μg NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-14-3-3 epsilon (TMAB-02078) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (TMAB-02078) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (TMAB-02078) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Testis tissue lysates Lane 5: Rat Placenta tissue lysates Lane 6: Human 293T cell lysates Lane 7: Human A375 cell lysates Lane 8: Human SW480 cell lysates Primary: Anti-14-3-3 epsilon (TMAB-02078) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 30 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Immunogen | KLH conjugated synthetic peptide: human 14-3-3E |
| Antigen Species | Human |
| Gene Name | YWHAE |
| Gene ID | |
| Protein Name | 14-3-3 protein epsilon |
| Uniprot ID | |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Molecular Weight | Theoretical: 29 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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