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Anti-14-3-3 beta/alpha Antibody (7X437)
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Catalog No. TMAC-00005
Alias YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β
Anti-14-3-3 beta/alpha Antibody (7X437) is a Rabbit antibody targeting 14-3-3 beta/alpha. Anti-14-3-3 beta/alpha Antibody (7X437) can be used in WB,IHC.
Anti-14-3-3 beta/alpha Antibody (7X437)
Anti-14-3-3 beta/alpha Antibody (7X437)
😃Good
Catalog No. TMAC-00005Alias YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β
Anti-14-3-3 beta/alpha Antibody (7X437) is a Rabbit antibody targeting 14-3-3 beta/alpha. Anti-14-3-3 beta/alpha Antibody (7X437) can be used in WB,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-14-3-3 beta/alpha Antibody (7X437) is a Rabbit antibody targeting 14-3-3 beta/alpha. Anti-14-3-3 beta/alpha Antibody (7X437) can be used in WB,IHC. |
| Alias | YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, β, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta, KCIP-1, HS1, HEL-S-1, GW128, 14-3-3 β |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of 14-3-3 on different lysates using anti-14-3-3 antibody at 1/1,000 dilution.Positive control: Lane 1: HT29, Lane 2: Human skin, Lane 3: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 antibody. Counter stained with hematoxylin. 3. Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle. 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon. 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10. Currently identified by mass spec as part of the TLR2 signaling complex and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 28 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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