Lucifer Yellow CH dilithium salt is a fluorescent dye commonly utilized for selective staining and studying the photodynamic disruption of eukaryotic and subcellular structures.

I. For selective cell staining
1. Cell preparation: Use cells cultured in appropriate culture medium. Lucifer Yellow CH Dilithium Salt is usually used in vitro to stain live or fixed cells.
2. Dye solution preparation: Dissolve Lucifer Yellow CH Dilithium Salt in an appropriate buffer (usually phosphate buffered saline (PBS)) at a concentration of 10-100 μM, depending on the experimental requirements.
3. Staining process: Add the dye solution to the cell culture and incubate for 10-30 minutes, or adjust the time according to the experimental conditions.
4. Post-treatment: After staining, it is usually necessary to wash with PBS to remove unbound dye.
5. Fluorescence detection: Use a fluorescence microscope (excitation wavelength: 430 nm, emission wavelength: 535 nm) to observe the stained cells or subcellular structures. Lucifer Yellow shows strong fluorescence for easy observation.
II. For photodynamic destruction studies
1. Cell treatment: After the cells are treated with Lucifer Yellow, irradiate the cells with light of an appropriate wavelength (usually UV or visible light).
2. Real-time imaging: Use fluorescence imaging technology to monitor the response of cells to photodynamic destruction and observe cell survival and structural changes.
III. Used to study endocytosis and membrane permeability
1. Staining process: Add the dye to the cell culture and incubate for 15-30 minutes, the specific time is adjusted according to the type of research.
2. Fluorescence microscopy: After staining, observe the staining pattern inside the cell under a fluorescence microscope.
IV. Used for neuronal communication and gap junction research
1. Neuronal staining: Lucifer Yellow can be used to mark neurons or other excitable cells to study their activity and communication.
2. Microscopic observation: Observe neuronal activity and intercellular communication under a fluorescence microscope.