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PI3K/Akt/mTOR signaling GSK-3 6-BIO

6-BIO

Catalog No. T1917   CAS 667463-62-9
Synonyms: BIO, MLS 2052, 6-Bromoindirubin-3'-oxime, GSK 3 Inhibitor IX, 6-bromoindirubin-3-oxime, GSK 3 IX
Purity 99.90% Datasheet

BIO is a specific inhibitor of GSK-3.

6-BIO, CAS 667463-62-9
Pack Size Availability Price/USD Quantity
2 mg In stock 50.00
5 mg In stock 84.00
10 mg In stock 116.00
25 mg In stock 267.00
50 mg In stock 483.00
100 mg In stock 876.00
1 mL * 10 mM (in DMSO) In stock 93.00
Bulk Size: Please Quote for Discount
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Purity 98.00%
Purity 98.00%
Biological Description
Chemical Properties
Storage & Solubility Information
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Description BIO is a specific inhibitor of GSK-3.
Targets&IC50 CDK1/CyclinB :ic50 0.32μM,   CDK2/CyclinA :ic50 0.30μM,   CDK5/p35 :ic50 0.08μM,   GSK-3 :ic50 5nM,   Tyk2 :ic50 30nM,  
Kinase Assay Kinase assay: Kinase activities are assayed in Buffer A or C at 30°C, at a final ATP concentration of 15 μM. Blank values are subtracted and activities calculated as pmoles of phosphate incorporated during a 10 min incubation. Controls are performed with appropriate dilutions of dimethylsulfoxide. In a few cases phosphorylation of the substrate is assessed by autoradiography after SDS-PAGE. GSK-3α/β is purified from porcine brain by affinity chromatography on immobilized axin. It is assayed, following a 1/100 dilution in 1 mg BSA/ml 10 mM DTT, with 5 μl 40 μM GS-1 peptide, a specific GSK-3 substrate, (YRRAAVPPSPSLSRHSSPHQSpEDEEE), in buffer A, in the presence of 15 μM [γ-32P] ATP (3,000 Ci/mmol; 1 mCi/ml) in a final volume of 30 μl. After 30 min incubation at 30°C, 25 μl aliquots of supernatant are spotted onto 2.5 × 3 cm pieces of Whatman P81 phosphocellulose paper, and 20 seconds later, the filters are washed five times (for at least 5 min each time) in a solution of 10 ml phosphoric acid/liter of water. The wet filters are counted in the presence of 1 ml ACS scintillation fluid.
Cell Research
COS1, Hepa (wild-type, CEM/LM AhR deficient and ELB1 ARNT deficient), or SH-SY5Y cells are grown in 6 cm culture dishes in Dulbecco's Modified Medium (DMEM) containing 10% fetal bovine serum. For treatment, IO (5 μM), BIO (5 or 10 μM), MeBIO (5 or 50 μM), LiCl (20 or 40 mM), or mock solution (DMSO, 0.5% final concentration) is added to medium when cell density reaches ∼70% confluence. After 12 (SH-SY5Y) or 24 hours, the cells, while still in plate, are lysed with lysis buffer (1% SDS, 1 mM sodium orthovanadate, 10 mM Tris [pH 7.4]). The lysate is passed several times through a 26G needle, centrifuged at 10,000 × g for 5 min, and adjusted to equal protein concentration. About 8 μg of each sample is loaded for immunoblotting. Enhanced chemiluminescence is used for detection. The following primary antibodies are used: mouse anti-β-catenin CT (Upstate Biotechnolgies, Clone 7D8, recognizes total β-catenin), mouse anti-phospho-β-catenin (Upstate Biotechnologies, Clone 8E7, recognizes dephosphorylated β-catenin), mouse anti-GSK-3 β, mouse anti-GSK-3 phosphoTyr216, rabbit anti-AhR (Aryl hydrocarbon receptor), and rabbit anti-actin.(Only for Reference)
Cell lines: COS1, Hepa or SH-SY5Y cells
Animal Research
Animal Model: mouse
Synonyms BIO, MLS 2052, 6-Bromoindirubin-3'-oxime, GSK 3 Inhibitor IX, 6-bromoindirubin-3-oxime, GSK 3 IX
Purity 99.34%
Appearance solid
Molecular Weight 356.17
Formula C16H10BrN3O2
CAS No. 667463-62-9

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

Ethanol: 21 mg/mL (58.96 mM)

DMSO: 71 mg/mL (199.34 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

30% PEG400+0.5% Tween80+5% propylene glycol: 30 mg/mL

Citations

References and Literature
1. Meijer L, et al. Chem Biol, 2003, 10(12), 1255-1266. 2. Sato N, et al. Nat Med, 2004, 10(1), 55-63. 3. Tseng AS, et al. Chem Biol, 2006, 13(9), 957-963. 4. Liu L, et al. Cancer Res. 2011 Jun 1;71(11):3972-3279.

Related Compound Libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Stem cell Differentiation Compound Library PI3K-AKT-mTOR Compound Library Tyrosine kinase inhibitor library DNA Damage & Repair Compound Library Kinase Inhibitor Library Anti-Cardiovascular Disease Compound Library Wnt&Hedgehog&Notch Compound Library Cell cycle related Compound Library

Related Products

Related compounds with same targets
Lithium citrate tribasic tetrahydrate Cyanoacetohydrazide AZD1080 LY2090314 AT 7519 hydrochloride salt AR-A014418 6-BIO AZD2858

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