VRK1 Protein, Human, Recombinant

Name: VRK1 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-04751
Price: 94 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: vaccinia related kinase 1, PCH1A, PCH1) Copy Product Info

Synonyms: vaccinia related kinase 1, PCH1A, PCH1

Catalog No. TMPY-04751 Copy Product Info

Purity: 95.3%

VRK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 45.6 kDa and the accession number is Q99986.

Pack Size	Price	USA Stock	Global Stock
5 µg	$94	-	In Stock
10 µg	$152	7-10 days	7-10 days
20 µg	$252	7-10 days	7-10 days
50 µg	$498	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:95.3%

Appearance:Solution

Color:Transparent

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Kinase activity untested
Description	VRK1 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 45.6 kDa and the accession number is Q99986.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	Q99986
Construction	A DNA sequence encoding the human VRK1 ( Q99986 ) (Met 1-Lys 396) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	vaccinia related kinase 1, PCH1A, PCH1
Research Background	VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.

Chemical Properties

Molecular Weight

45.6 kDa (predicted); 47 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	Proteins are shipped with blue ice.
Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.