VEGF164 Protein, Mouse, Recombinant (E. coli)

Name: VEGF164 Protein, Mouse, Recombinant (E. coli)
Brand: TargetMol
SKU: TMPS-00178
Rating: 4.5 (1 reviews)

(Synonyms: VPF, VEGF-164, Vascular Permeability Factor, Glioma-derived endothelial cell mitogen, Folliculostellate cell-derived growth factor) Copy Product Info

Synonyms: VPF, VEGF-164, Vascular Permeability Factor, Glioma-derived endothelial cell mitogen, Folliculostellate cell-derived growth factor

Catalog No. TMPS-00178 Copy Product Info

VEGF164 Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The accession number is Q00731-2.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$117	7-10 days	7-10 days
10 µg	$192	7-10 days	7-10 days
20 µg	$273	7-10 days	7-10 days
50 µg	$438	7-10 days	7-10 days
100 µg	$686	7-10 days	7-10 days
200 µg	$1,120	7-10 days	7-10 days
500 µg	$2,080	7-10 days	7-10 days
1 mg	$3,390	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	The ED 50 < 5.0 ng/ml, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2.0 × 10 5 units/mg.
Description	VEGF164 Protein, Mouse, Recombinant (E. coli) is expressed in E. coli. The accession number is Q00731-2.
Species	Mouse
Expression System	E. coli
Tag	Tag Free
Accession Number	Q00731-2
Construction	Ala27-Arg190
Protein Purity	> 98% as determined by SDS-PAGE
Endotoxin	< 0.2 EU/μg of protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	VPF, VEGF-164, Vascular Permeability Factor, Glioma-derived endothelial cell mitogen, Folliculostellate cell-derived growth factor
Research Background	Vascular Endothelial Growth Factor (VEGF) was initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and by a variety of transformed cell lines as a mitogen specific for vascular endothelial cells. It was subsequently found to be identical to an independently discovered vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based on its ability to increase the permeability of capillary blood vessels. Three mouse cDNA clones, which arise through alternative splicing and which encode mature mouse monomeric VEGF having 120, 164, or 188, amino acids, respectively, have been identified. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 (the mouse homologue of human KDR), both members of the type III subclass of RTKs containing seven immunoglobulin-like repeats in their extracellular domains, have been shown to bind VEGF with high affinity. The roles of the homodimers of KDR, Flt, and the heterodimer of KDR/Flt in VEGF signal transduction remain to be elucidated. In vivo, VEGF has been found to be a potent angiogenesis inducer.

Chemical Properties

Molecular Weight

~19.4 kDa (Reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.